ERHART, TEMAN*, MARK V. WILSON, AND AARON LISTON. Department of Botany & Plant Pathology, Oregon State University, Corvallis, Oregon 97330. - Population dynamics and conservation biology of Lupinus sulphureus ssp. kincaidii (Fabaceae).
Kincaidís lupine (Lupinus sulphureus ssp. kincaidii
(Smith) Phillips) is a rare endemic plant of the Willamette Valley.
This member of the Fabaceae is a long-lived perennial plant of native
upland prairie remnants. It was listed as a Threatened species under the
Endangered Species Act in January of 2000. It is also a nearly obligate
larval foodplant of a rare butterfly, Fenderís blue butterfly, which
was concurrently listed as an Endangered species. Low seed production
and progeny fitness has been observed in many populations. This is
suspected to be a result of possible self-incompatibility and inbreeding
depression in small isolated populations. As part of a study of
Kincaidís lupine biology, the effects of gamete source on seed
production and progeny fitness were tested, with hand pollination
treatments, in two natural populations. Pollination treatments were
used as an indirect method to assess the effects of selfing versus
crossing across various genetic distances. Inter-simple sequence repeat (ISSR)
markers are also being used as a PCR-based method to genotype sampled
plants in the gamete source experiment. These data will be used to
calculate the genetic distance between individuals used in the crossing
treatments, and these values will be correlated with seed set and
progeny performance. These molecular markers will also allow estimation of
genetic diversity, and may indicate population viability concerns.
The extent of clonal growth and spread in the two study populations
will also be documented.
Key words: endemic, hand pollination, inter-sequence-series repeat, Kincaid's, lupine, Lupinus sulphureus ssp. kincaidii