Buxaceae are a small eudicot family of mostly monoecious evergreen shrubs and comprise up to five genera (Buxus, Notobuxus, Sarcococca, Pachysandra, Styloceras) and 120 species. Delimination of the family and inter- and intrageneric relationships within the family have been controversial. Sequences of nuclear ITS and plastid ndhF from 25 representatives of Buxaceae were analyzed separately and in combination to resolve phylogenetic relationships among and within genera. Two major clades were identified: the clade of Pachysandra, Sarcococca, and Styloceras and the clade of Buxus andNotobuxus. Pachysandra and Sarcococca were two strongly supported monophyletic groups. The sister relationship of Sarcococca to the clade of Pachysandra and Styloceras is also strongly supported. American and Eurasian Buxus each form a strongly supported clade. The controversial genus Notobuxus is embedded among African members of Buxus. Ongoing morphological studies will improve our understanding of the evolution of morphological characters and phylogenetic relationships of Buxaceae.

Key words: Buxaceae, character evolution, ITS, molecular phylogeny, ndhF