BELL, CHARLES D.* AND MICHAEL J. DONOGHUE. Harvard University Herbaria, 22 Divinity Avenue, Cambridge MA, 02138 and Department of Ecology and Evolutionary Biology, Yale University, New Haven, CN 06511. - Dipsacales phylogeny based on chloroplast DNA sequences.
Ten new DNA sequences of the 5’ end of the chloroplast ndhF
gene, along with 5 new rbcL sequences, were obtained for taxa
representing Caprofoliaceae, Valerianaceae, and Dipsacaceae. These
were combined with 31 published ndhF sequences, as well as
published rbcL sequences and morphological characters.
Phylogenetic relationships were estimated using parsimony and maximum
likelihood methods. Trees inferred from all datasets and methods are
in strong agreement with previous hypotheses based on morphology,
rbcL, and combined datasets (M. Donoghue, T. Eriksson, P.
Reeves, and R. Olmstead, manuscript in review). There is strong
support for the monophyly of traditional Dipsacales and for Adoxaceae
(including Viburnum, Sambucus, and Adoxa) and
Caprifoliaceae (including Morinaceae, Valerianaceae, and Dipsacaceae).
The new sequences also provide additional support for lineages within
Adoxaceae and Caprifoliaceae. For example, Tetradoxa and
Sinadoxa are related to Adoxa, and Linnaeeae are
monophyletic and united with a clade including Morinaceae,
Valerianaceae, and Dipsacaceae. We also generated sequence data from
several additional chloroplast genes to help resolve phylogenetic
relationships within Morinaceae (Morina, Acanthocalyx,
and Cryptothladia) and Caprifolieae (Leycesteria,
Lonicera, Symphoricarpus, and Triosteum).
Key words: chloroplast genes, Dipsacales, Morinaceae, ndhF, phylogeny, rbcL